Antigen

Antigen Class

TFs and others

Antigen

Vsx2

Cell type

Cell type Class

Neural

Cell type

Retina

MeSH Description

The ten-layered nervous tissue membrane of the eye. It is continuous with the OPTIC NERVE and receives images of external objects and transmits visual impulses to the brain. Its outer surface is in contact with the CHOROID and the inner surface with the VITREOUS BODY. The outer-most layer is pigmented, whereas the inner nine layers are transparent.

Sample

source_name

Adult mouse retina

developmental stage

Adult

tissue

retina

strain

C57BL/6

chip antibody

VSX2 (Exalpha, Cat No. X1180P, Lot No 14166)

treatment

untreated

Sequenced DNA Library

library_name

GSM5860055

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Retinae were fixed, lysed and sheared to an average length of 300-500 bp. After incubation with antibody, beads were washed and DNA was extracted with phenol chroloform and precipitated with ethanol. Libraries were prepared according to Illumina's instructions

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

38479041

Reads aligned (%)

94.6

Duplicates removed (%)

27.6

Number of peaks

31415 (qval < 1E-05)

mm9

Number of total reads

38479041

Reads aligned (%)

94.5

Duplicates removed (%)

27.7

Number of peaks

31399 (qval < 1E-05)